Imuran

Azathioprine.

Each tablet contains 50 mg of the active ingredient azathioprine.
Excipients/Inactive Ingredients: Tablets: Core tablet: lactose monohydrate, maize starch, pregelatinised starch, magnesium stearate, stearic acid, purified water.
Film coating: hypromellose, macrogol 400, purified water.

Pharmacology: Pharmacodynamics: Mechanism of Action: Azathioprine is an imidazole derivate of 6-mercaptopurine (6-MP). It is rapidly broken down in vivo into 6-MP and a methylnitroimidazole moiety. The 6-MP readily croses cell membranes and is converted intracellulary into a number of purine thioanalogues, which include the main active nucleotide, thioinosinic acid. The rate of conversion varies from one person to another. Nucleotides do not traverse cell membranes and therefore do not circulate in body fluids. Irrespective of whether it is given directly or is derived in vivo from azathioprine, 6-MP is eliminated mainly as the inactive oxidised metabolite thiouric acid. This oxidation is brought about by xanthine oxidase, an enzyme that is inhibited by allopurinol. Other potential mechanisms of azathioprine include the inhibition of many pathways in nucleic acid biosynthesis, hence preventing proliferation of cells involved in determination and amplification of the immune response. Because of these mechanisms, the therapeutic effect of azathioprine may be evident only after several weeks or months of treatment.
The activity of the methylnitroimidazole moiety, a metabolite of azathioprine but not 6-MP, has not been defined clearly. However, in several systems it appears to modify the activity of azathioprine as compared with that of 6-MP.
Pharmacodynamic Effects: Plasma levels of azathioprine and 6-MP do not correlate well with the therapeutic efficacy or toxicity of azathioprine, and therefore have no prognostic value.
Pharmacokinetics: Absorption: The absorption of azathioprine is incomplete and variable. The median (range) absolute bioavailability of 6-MP after administration of azathioprine 50 mg is 47% (27-80%). The extent of absorption of azathioprine is similar across the gastrointestinal tract, including the stomach, jejunum, and cecum. However, the extent of 6-MP absorption, after azathioprine administration is variable and differs between the sites of absorption, with the highest extent of absorption in the jejunum, followed by the stomach and then the cecum.
Although there are no food effect studies with azathioprine, pharmacokinetic studies with 6-MP have been conducted that are relevant to azathioprine. The mean relative bioavailability of 6-MP was approximately 26% lower following administration with food and milk compared to an overnight fast. 6-MP is not stable in milk due to the presence of xanthine oxidase (30% degradation within 30 minutes). Azathioprine should be administered at least one hour before or three hours after food or milk.
Distribution: The volume of distribution at steady state (Vdss) of azathioprine is unknown. The mean (±SD) apparent Vdss of 6-MP is 0.9 (±0.8) L/kg, although this may be an underestimate because 6-MP is cleared throughout the body (and not just in the liver).
It is predicted that 30% of azathioprine is protein bound.
Concentrations of 6-MP in cerebrospinal fluid (CSF) are low or negligible after IV or oral administration of 6-MP.
Special Patient Populations: Elderly: No specific studies have been carried out in the elderly.
Overweight paediatric population: In a US clinical study, 18 children (aged 3 to 14 years) were evenly divided into two groups; either a weight to height ratio above or below the 75^th percentile. Each child was on maintenance treatment of 6-MP and the dosage was calculated based on their body surface area. The mean AUC (0-∞) of 6-MP in the group above the 75th percentile was 2.4 times lower than that for the group below the 75^th percentile. Therefore, children considered to be overweight may require azathioprine doses at the higher end of the dose range and close monitoring of response to treatment is recommended.
Renal Impairment: It has been suggested that the toxicity of azathioprine may be enhanced in the presence of renal insufficiency, but controlled studies have not supported this suggestion. Nevertheless, it is recommended that the dosage used should be at the lower end of the normal range and the haematological response should be carefully monitored. Dosage should be further reduced if haematological toxicity occurs.
Azathioprine and/or its metabolites are eliminated by haemodialysis, with approximately 45% of radioactive metabolites eliminated during dialysis of eight hours.
Hepatic Impairment: A study with azathioprine was performed in three groups of renal transplant patients: those without liver disease, those with hepatic impairment (but no cirrhosis) and those with hepatic impairment and cirrhosis. The study demonstrated that 6-mercaptopurine exposure was 1.6 times higher in patients with hepatic impairment (but no cirrhosis) and six times higher in patients with hepatic impairment and cirrhosis, compared to patients without liver disease. Therefore, consideration should be given to reducing the dosage in patients with impaired hepatic function (see Dosage & Administration).
Toxicology: Pre-clinical Safety Data: Mutagenicity: Azathioprine was found to be mutagenic in a number of in vitro and in vivo genotoxicity assays.
Carcinogenicity: Long-term carcinogenicity studies of azathioprine showed an increased incidence of lymphosarcomas, as well as epithelial tumours and carcinomas in mice and rats, respectively, at dosages of up to 2-fold the human therapeutic dose and at lower dosages in immunocompromised mice.
Reproductive Toxicology: Studies in pregnant rats, mice and rabbits using azathioprine in dosages from 5 to 15 mg/kg bodyweight/day over the period of organogenesis have shown varying degrees of foetal abnormalities.
Teratogenicity was evident in rabbits at 10 mg/kg body weight/day.

Imuran is indicated for the treatment of patients who have received an organ transplant.
Imuran has a significant therapeutic effect in a proportion of patient suffering from chronic active hepatitis, severe rheumatoid arthritis, systemic lupus erythematosus (SLE), dermatomyositis, pemphigus vulgaris, polyarteritis nodosa, acquired hemolytic anemia, idiopathic thrombocytopenic purpura.
Imuran is used in conditions other than transplantation, when: they are refractory to corticosteroids alone, or they are controlled by corticosteroids in dosages producing or likely to produce severe side effects and the primary aim is to reduce the requirements of steroids; or corticosteroids are contra-indicated.
In pemphigus and rheumatoid arthritis, Imuran has been shown to have significant therapeutic activity when used without corticosteroids. In other conditions or where local registration authority approval has not yet been given for a particular condition, the use of Imuran must be regarded as experimental. The risk associated with therapy with Imuran should be considered against the severity of the patient's condition and the prospects of therapeutic benefit.

Adults and Children: In Transplants: In renal transplantation the dose of Imuran required to prevent rejection and minimize toxicity will vary with individual patients; this necessitates careful management. Initial dose is usually 3 to 5 mg/kg daily, beginning at the time of transplant. Imuran is usually given as a single daily dose on the day of, and in a minority of cases to three days before transplantation. Imuran is often initiated with the intravenous administration of the sodium salt, with the subsequent use of film-coated tablets (at the same dose level) after the post-operative period. Intravenous administration of the sodium salt is indicated only in patients unable to tolerate oral medications. Dose reduction to maintenance levels of 1 to 3 mg/kg daily is usually possible. The dose of Imuran should not be increased to toxic levels because of threatened rejection. Discontinuation may be necessary for severe haematologic or other toxicity, even if rejection of the hemograft may be a consequence of drug withdrawal.
Therapy with Imuran should be maintained indefinitely unless positive contra-indications should arise. Sometimes only a very low dose is necessary if therapy is discontinued even after a period of years, there is a high risk of graft rejection within a few weeks. In other conditions, the dosage and duration of treatment may vary according to the condition, its severity and the clinical response obtained.
A therapeutic response may not be evident a few days or even weeks after initiation of therapy. If no improvement occurs in the patient's condition within three months, consideration should be given to withdrawing Imuran.
For the treatment of most conditions the starting dose is 2 to 2.5 mg/kg orally which should be reduced if haematological or other complications occur.
In chronic active hepatitis the dose is 1 to 1.5 mg/kg/day orally. In rheumatoid arthritis lower doses can be effective.

Symptoms: Unexplained infection, ulceration of the throat, bruising and bleeding are the main signs of overdosage with azathioprine and result from bone marrow depression, which may be maximal after nine to 14 days. These signs are more likely to be manifest following chronic overdosage, rather than after a single acute overdose. There has been a report of a patient who ingested a single overdose of 7.5 g of azathioprine. The immediate toxic effects of this overdose were nausea, vomiting and diarrhoea, followed by mild leukopenia and mild abnormalities in liver function. Recovery was uneventful.
Management: As there is no specific antidote, blood counts should be closely monitored and general supportive measures, together with appropriate blood transfusion, instituted if necessary. Active measures (such as the use of activated charcoal) may not be effective in the event of azathioprine overdose unless the procedure can be undertaken within 60 minutes of ingestion.
Further management should be as clinically indicated or as recommended by the national poisons centre, where available. The value of dialysis in patients who have taken an overdose of azathioprine is not known, though azathioprine is partially dialyzable.
Symptomatic treatment should be administered. Azathioprine is known to be dialyzable and dialysis might be considered in severe cases. Subsequent monitoring, including haematological monitoring, is necessary to allow prompt treatment of any adverse effects which may develop.

Contraindicated in patients known to be hypersensitive to the active substance azathioprine or to any of the excipients listed in Description. Hypersensitivity to 6-mercaptopurine should alert the prescriber to probable hypersensitivity to azathioprine.

Immunisation using a live organism vaccine has the potential to cause infection in immunocompromised hosts. Therefore, it is recommended that patients do not receive live organism vaccines until at least three months after the end of their treatment with azathioprine (see Interactions).
Co-administration of ribavirin and azathioprine is not advised. Ribavirin may reduce efficacy and increase toxicity of azathioprine (see Interactions).
Monitoring: There are potential hazards to the use of azathioprine. It should be prescribed only if the patient can be adequately monitored for toxic effects throughout the duration of therapy.
Particular care should be taken to monitor haematological response and to reduce the maintenance dosage to the minimum required for clinical response.
It is suggested that during the first eight weeks of therapy, complete blood counts, including platelets, should be performed weekly or more frequently if high dosage is used or if severe renal and/or hepatic disorder is present. The blood count frequency may be reduced later in therapy, but it is suggested that complete blood counts are repeated monthly or at least at intervals of not longer than three months.
At the first signs of an abnormal fall in blood counts, treatment should be interrupted immediately as leucocytes and platelets may continue to fall after treatment is stopped.
Azathioprine may be given long-term unless the patient cannot tolerate the preparation. Withdrawal of an effective dose in certain instances eg, SLE with nephritis, may result in a serious relapse of the condition.
In other instances such as rheumatoid arthritis and certain hematological conditions, treatment may be withdrawn after a suitable interval without any ill-effect. Withdrawal should always be a gradual process performed under close supervision.
In the presence of severe renal or hepatic impairment, careful monitoring is initially required, since the dosage of azathioprine may have to be reduced.
Patients receiving azathioprine should be instructed to report immediately any evidence of infection, unexpected bruising or bleeding or other manifestations of bone marrow depression. Bone marrow suppression is reversible if azathioprine is withdrawn early enough.
Azathioprine is hepatotoxic and liver function tests should be routinely monitored during treatment. More frequent monitoring may be advisable in those with pre-existing liver disease or receiving other potentially hepatotoxic therapy. The patient should be instructed to discontinue azathioprine immediately if jaundice becomes apparent.
There are individuals with an inherited deficiency of the enzyme thiopurine methyltransferase (TPMT) who may be unusually sensitive to the myelosuppresive effect of azathioprine and prone to developing rapid bone marrow depression following the initiation of treatment with azathioprine. This problem could be exacerbated by co-administration with drugs that inhibit TPMT such as olsalazine, mesalazine or sulphasalazine. Also a possible association between decreased TPMT activity and secondary leukaemias and myelodysplasia has been reported in individuals receiving 6-mercaptopurine (the active metabolite of azathioprine) in combination with other cytotoxics (see Adverse Reactions). Some laboratories offer testing for TPMT deficiency, although these tests have not been shown to identify all patients at risk of severe toxicity. Therefore close monitoring of blood counts is still necessary. When possible, concomitant administration of cytostatic drugs or drugs which may have a myelosuppression effect should be avoided.
Hypersensitivity: Patients suspected to have previously presented a hypersensitivity reaction to 6-mercaptopurine should not be recommended to use its pro-drug azathioprine, and vice-versa, unless the patient has been confirmed as hypersensitive to the culprit drug with allergological tests, and tested negative for the other.
Infection: Severe secondary infections, often with uncommon organisms are a hazard of immunosuppressive therapy. These are seen more frequently in transplant recipients than in patients being treated for other indications.
Mutagenicity: Chromosomal abnormalities have been demonstrated in both male and female patients treated with azathioprine. It is difficult to assess the role of azathioprine in the development of these abnormalities.
Chromosomal abnormalities, which disappear with time, have been demonstrated in lymphocytes from the off-spring of patients treated with azathioprine. Except in extremely rare cases, no overt physical evidence of abnormality has been observed in the offspring of patients treated with azathioprine.
Azathioprine and long-wave ultraviolet light have been shown to have a synergistic clastogenic effect in patients treated with azathioprine for a range of disorders.
Teratogenicity: The potential teratogenicity of azathioprine should be borne in mind. Although it has been shown to be teratogenic in laboratory animals clinical evidence suggests that the risk is not appreciable in man. There is no doubt that azathioprine and its metabolites cross the placenta. A temporary impairment of immune function has been noted following exposure in utero to azathioprine combined with prednisone. The long-term consequences of these properties of azathioprine are unknown, but many children exposed in utero have now completed the first decade of life without reported problems.
Carcinogenicity: An increased number of malignant tumours, especially lymphoreticular and epithelial, has been observed in transplant recipients. The skin tumours that have occurred in transplant patients have been primarily on sun exposed skin. Patients should be cautioned against undue sun exposure and the skin should be examined at regular intervals. There is, however, as yet no conclusive evidence of an increased incidence of tumours in other azathioprine treated subjects. In such patients, the risk may be indistinguishable from that accompanying some of the diseases under treatment.
The few cases reported show a different pattern from that seen in transplantation; tumour occurrence is much less common, has an increased latency, is seen mainly after prolonged continuous therapy, is less exclusively lymphoreticular and tends to occur in those patients also treated with alkylating agents.
Patients receiving immunosuppressive therapy, including azathioprine are at an increased risk of developing lymphoproliferative disorders and other malignancies, notably skin cancers (melanoma and non-melanoma), sarcomas (Kaposi's and non-Kaposi's) and uterine cervical cancer in situ. The increased risk appears to be related to the degree and duration of immunosuppression. It has been reported that discontinuation of immunosuppression may provide partial regression of the lymphoproliferative disorder.
A treatment regimen containing multiple immunosuppressants (including thiopurines) should therefore be used with caution as this could lead to lymphoproliferative disorders, some with reported fatalities. A combination of multiple immunosuppressants, given concomitantly increases the risk of Epstein-Barr virus (EBV)-associated lymphoproliferative disorders.
Reports of hepatosplenic T-cell lymphoma have been received when azathioprine is used alone or in combination with anti-TNF agents or other immunosuppressants. Although most reported cases occurred in the IBD population, there have also been cases reported outside of this population.
Patients receiving multiple immunosuppressive agents may be at risk of over-immunosuppression, therefore such therapy should be maintained at the lowest effective level. As is usual for patients with increased risk for skin cancer, exposure to sunlight and UV light should be limited, and patients should wear protective clothing and use a sunscreen with a high protection factor.
Macrophage Activation Syndrome: Macrophage Activation Syndrome (MAS) is a known, life-threatening disorder that may develop in patients with autoimmune conditions, in particular with autoimmune conditions, in particular with Inflammatory Bowel Disease (IBD), and there could potentially be an increased susceptibility for developing the condition with the use of azathioprine. If MAS occurs, or is suspected, evaluation and treatment should be started as early as possible, and treatment with azathioprine should be discontinued. Physicians should be attentive to symptoms of infection such as EBV and cytomegalovirus (CMV), as these are known triggers for MAS.
Varicella Zoster Virus Infection (see Adverse Reactions): Infection with Varicella Zoster Virus (VZV; chickenpox and herpes zoster) may become severe during the administration of immunosuppressants. Caution should be exercised especially with respect to the following: Before starting the administration of immunosuppressants, the prescriber should check to see if the patient has a history of VZV. Serologic testing may be useful in determining previous exposure. Patients who have no history of exposure should avoid contact with individuals with chickenpox or herpes zoster. If the patient is exposed to VZV, special care must be taken to avoid patients developing chickenpox or herpes zoster, and passive immunisation with Varicella-Zoster Immunoglobulin (VZIG) may be considered.
If the patient is infected with VZV, appropriate measures should be taken, which may include antiviral therapy and supportive care.
Progressive Multifocal Leukoencephalopathy (PML): PML, an opportunistic infection caused by the JC virus, has been reported in patients receiving azathioprine with other immunosuppressive agents. Immunosuppressive therapy should be withheld at the first sign or symptoms suggestive of PML and appropriate evaluation undertaken to establish a diagnosis (see Adverse Reactions).
Hepatitis B (see Adverse Reactions): Hepatitis B carriers (defined as patients positive for hepatitis B surface antigen [HBsAg] for more than six months), or patients with documented past HBV infection, who receive immunosuppressive drugs are at risk of reactivation of HBV replication, with asymptomatic increases in serum HBV DNA and ALT levels. Local guidelines may be considered including prophylactic therapy with oral anti-HBV agents.
Xanthine Oxide Inhibitors: If allopurinol, oxipurinol and/or thipurinol are given concomitantly with azathioprine, the dosage of azathioprine must be reduced to a quarter of the original dose (see Interactions).
Neuromuscular Agents: Special care is necessary when azathioprine is given concomitantly with neuromuscular acting agents like tubucurarine or succinylcholine (see Interactions). It can also potentiate the neuromuscular block that is produced by depolarising agents such as succinylcholine (see Interactions). Patients should be advised to inform their anaesthesiologist of their treatment with azathioprine prior to surgery.
Effects on the Ability to Drive and Use Machines: There are no data on the effect of azathioprine on driving performance or the ability to operate machinery. A detrimental effect on these activities cannot be predicted from the pharmacology of the drug.
Renal and/or Hepatic Impairment: Caution is advised during the administration of azathioprine in patients with renal impairment and/or hepatic impairment. Consideration should be given to reducing the dosage in these patients and haematological response should be carefully monitored (see Dosage & Administration and Pharmacokinetics under Actions).

Fertility: Relief of chronic renal insufficiency by renal transplantation involving the administration of azathioprine has been accompanied by improved fertility in both male and female transplant recipients.
The specific effect of azathioprine therapy on human fertility is unknown but there are reports of successful fatherhood/motherhood after receiving treatment. Several studies report that azathioprine at standard doses does not appear to affect male fertility.
Pregnancy: Substantial transplacental and transamniotic transmission of azathioprine and its metabolites from the mother to the foetus have been shown to occur.
Azathioprine should not be given to patients who are pregnant or likely to become pregnant in the near future without careful assessment of risk versus benefit.
Evidence of the teratogenicity of azathioprine in man is equivocal. As with all cytotoxic chemotherapy, adequate contraceptive precautions should be advised when either partner is receiving azathioprine.
There have been reports of intra-uterine growth retardation, premature birth and low birth weight following maternal exposure to azathioprine, particularly in combination with corticosteroids. There have also been reports of spontaneous abortion following either maternal or paternal exposure.
Leukopenia and/or thrombocytopenia have been reported in a proportion of neonates whose mothers took azathioprine throughout their pregnancies. Extra care in haematological monitoring is advised during pregnancy.
Breastfeeding: 6-Mercaptopurine has been identified in the colostrum and breast-milk of women receiving azathioprine treatment. It is recommended that mothers receiving azathioprine should not breastfeed.

Summary of The Safety Profile: For this product there is no modern clinical documentation which can be used as support for determining the frequency of undesirable effects. Undesirable effects may vary in their incidence depending on the indication. The following convention has been utilised for the classification of frequency: very common (≥1/10); common (≥1/100 to <1/10); uncommon (≥1/1,000 to <1/100); rare (≥1/10,000 to <1/1,000); very rare (<1/10,000); not known: cannot be estimated from the available data. See table.


Click on icon to see table/diagram/image


Description of Selected Adverse Reactions: Infections and Infestations: Patients receiving azathioprine alone or in combination with other immunosuppressants, particularly corticosteroids, have shown increased susceptibility to viral, fungal and bacterial infections, including severe or atypical infection and reactivation with VZV, hepatitis B and other infectious agents.
Neoplasms benign and malignant (including cysts and polyps): Patients receiving immunosuppressive therapy, including azathioprine are at an increased risk of developing lymphoproliferative disorders and other malignancies, notably skin cancers (melanoma and non-melanoma), sarcomas (Kaposi’s and non-Kaposi’s) and uterine cervical cancer in situ. The increased risk appears to be related to the degree and duration of immunosuppression. It has been reported that discontinuation of immunosuppression may provide partial regression of the lymphoproliferative disorder.
There have been rare reports of acute myeloid leukaemia and myelodysplasia (some in association with chromosomal abnormalities).
Blood and Lymphatic System Disorders: Azathioprine may be associated with a dose-related, generally reversible, depression of bone marrow function, most frequently expressed as leukopenia, but also sometimes as anaemia and thrombocytopenia and rarely as agranulocytosis, pancytopenia and aplastic anaemia. These occur particularly in patients predisposed to myelotoxicity, such as those with TPMT deficiency and renal or hepatic insufficiency and in patients failing to reduce the dose of azathioprine when receiving concurrent allopurinol therapy.
Reversible, dose-related increases in mean corpuscular volume and red cell haemoglobin content have occurred in association with azathioprine therapy. Megaloblastic bone marrow changes have also been observed but severe megaloblastic anaemia and erythroid hypoplasia are rare.
Immune System Disorders: Several different clinical syndromes, which appear to be idiosyncratic manifestations of hypersensitivity, have been described occasionally following administration of azathioprine. Clinical features include general malaise, dizziness, nausea, vomiting, diarrhoea, fever, rigors, exanthema, rash, vasculitis, myalgia, arthralgia, hypotension, renal dysfunction, hepatic dysfunction and cholestasis (see Adverse Reactions).
In many cases, rechallenge has confirmed an association with azathioprine.
Immediate withdrawal of azathioprine and institution of circulatory support where appropriate have led to recovery in the majority of cases.
Other marked underlying pathology has contributed to the very rare deaths reported.
Following a hypersensitivity reaction to azathioprine, the necessity for continued administration of azathioprine should be carefully considered on an individual basis.
Gastrointestinal Disorders: Some patients experience nausea when first given azathioprine. With oral administration, nausea appears to be relieved by administering the tablets after meals. However administration of azathioprine tablets after meals may reduce oral absorption, therefore monitoring for therapeutic efficacy should be considered after administration in this way (see Pharmacokinetics under Actions).
Serious complications, including colitis, diverticulitis and bowel perforation, have been described in transplant recipients receiving immunosuppressive therapy. However, the aetiology is not clearly established and high-dose corticosteroids may be implicated. Severe diarrhoea, recurring on rechallenge, has been reported in patients treated with azathioprine for inflammatory bowel disease. The possibility that exacerbation of symptoms might be drug-related should be borne in mind when treating such patients.
Pancreatitis has been reported in a small percentage of patients on azathioprine therapy, particularly in renal transplant patients and those diagnosed as having inflammatory bowel disease. There are difficulties in relating the pancreatitis to the administration of one particular drug, although rechallenge has confirmed an association with azathioprine on occasions.
Hepatobiliary Disorders: Cholestasis and deterioration of liver function have occasionally been reported in association with azathioprine therapy and are usually reversible on withdrawal of therapy. This may be associated with symptoms of a hypersensitivity reaction (see Adverse Reactions).
Rare, but life-threatening hepatic damage associated with chronic administration of azathioprine has been described primarily in transplant patients. Histological findings include sinusoidal dilatation, peliosis hepatis, veno-occlusive disease and nodular regenerative hyperplasia. In some cases withdrawal of azathioprine has resulted in either a temporary or permanent improvement in liver histology and symptoms.
Skin and Subcutaneous Tissue Disorders: Hair loss has been described on a number of occasions in patients receiving azathioprine and other immunosuppressive agents. In many instances the condition resolved spontaneously despite continuing therapy. The relationship between alopecia and azathioprine treatment is uncertain.

Vaccines: The immunosuppressive activity of azathioprine could result in an atypical and potentially deleterious response to live vaccines it is therefore recommended that patients do not receive live vaccines until at least three months after the end of their treatment with azathioprine (see Precaustions).
A diminished response to killed vaccines is likely and such a response to hepatitis B vaccine has been observed among patients treated with a combination of azathioprine and corticosteroids.
A small clinical study has indicated that standard therapeutic doses of azathioprine do not deleteriously affect the response to polyvalent pneumococcal vaccine, as assessed on the basis of mean anti-capsular specific antibody concentration.
Effect of Concomitant Drugs on Azathioprine: Ribavirin: Ribavirin inhibits the enzyme, inosine monophosphate dehydrogenase (IMPDH), leading to a lower production of the active 6-thioguanine nucleotides. Severe myelosuppression has been reported following concomitant administration of azathioprine and ribavirin; therefore co-administration is not advised (see Precautions).
Cytostatic/Myelosuppressive Agents (see Precautions): Where possible, concomitant administration of cytostatic drugs, or drugs which may have a myelosuppressive effect, such as penicillamine, should be avoided. There are conflicting clinical reports of interactions, resulting in serious haematological abnormalities, between azathioprine and trimethoprim/sulfamethoxazole (co-trimoxazole).
There have been case reports suggesting that haematological abnormalities may develop due to the concomitant administration of Imuran and ACE inhibitors.
It has been suggested that cimetidine and indomethacin may have myelosuppressive effects which may be enhanced by concomitant administration of azathioprine.
Allopurinol/Oxipurinol/Thiopurinol and Other Xanthine Oxidase Inhibitors: Xanthine oxidase activity is inhibited by allopurinol, oxipurinol and thiopurinol, which results in reduced conversion of biologically active 6-thioinosinic acid to biologically inactive 6-thiouric acid. When allopurinol, oxipurinol and/or thiopurinol are given concomitantly with 6-mercaptopurine or azathioprine, the dose of 6-mercaptopurine and azathioprine should be reduced to 25% of the original dose (see Precautions).
Other xathine oxidase inhibitors, such as febuxostat may decrease the metabolism of azathioprine. Concomitant administration is not recommended as data are insufficient to determine an adequate dose reduction.
Concomitant Use of Muscle Relaxants: There is clinical evidence that azathioprine antagonizes the effect of non-depolarising muscle relaxants such as curare, d-tubocurarine and pancuronium. Experimental data confirm that azathioprine reverses the neuromuscular blockade caused by d-tubocurarine, and show that azathioprine potentiates the neuromuscular blockage caused by succinylcholine.
Aminosalicylates: There is in vitro and in vivo evidence that aminosalicylate derivatives (e.g. olsalazine, mesalazine or sulphasalazine) inhibit the TPMT enzyme. Therefore, lower doses of azathioprine may need to be considered when administered concomitantly with aminosalicylate derivatives (see Precautions).
Infliximab: An interaction has been observed between azathioprine and infliximab. Patients receiving ongoing azathioprine experienced transient increases in 6-TGN (6-thioguanine nucleotide, an active metabolite of azathioprine) levels and a decerease in the mean leukocyte count in the initial weeks following infliximab infusion, which returned to previous levels after three months.
Neuromuscular agents: There is clinical evidence that azathioprine antagonizes the effect of non-depolarising muscle relaxants such as curare, d-tubocurarine and pancuronium. Experimental data confirm that azathioprine reverses the neuromuscular blockade produced by d-tubocurarine, and show that azathioprine potentiates the neuromuscular blockade produced by succinylcholine (see Precautions).
Effect of Azathioprine on Other Drugs: Anticoagulants: Inhibition of the anticoagulant effect of warfarin and acenocoumarol has been reported when co-administered with azathioprine; therefore higher doses of the anticoagulant may be needed. It is recommended that coagulation tests are closely monitored when anticoagulants are concurrently administered with azathioprine.

Instructions for Use/Handling: Safe Handling: Health professionals who handle uncoated azathioprine tablets should follow guidelines for the handling of cytotoxic drugs according to prevailing local recommendations and/or regulations.
Provided that the film-coating is intact, there is no risk in handling film-coated azathioprine tablets. Film-coated azathioprine tablets should not be divided and, provided the coating is intact, no additional precautions are required when handling them.
Disposal: Azathioprine tablets should be disposed of in a manner appropriate to the prevailing local regulatory requirements for the destruction of dangerous substances.
Incompatibilities: None reported for Imuran tablets.

Store below 30°C. Protect from light.

Disease-Modifying Anti-Rheumatic Drugs (DMARDs) / Immunosuppressants

L04AX01 - azathioprine ; Belongs to the class of other immunosuppressants.

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