Store between 2-8°C but not allowed to freeze. When in use, vial may be kept at room temperature (15°C to 25°C) for up to six weeks. Do not expose to excessive heat or direct sunlight. Insulin preparations which have been frozen must not be used. Once opened (when the stopper or seal has been punctured with a needle), insulin is kept at room temperature. Cold insulin can be irritating to inject. Thus, patients should be asked to roll the vial in their hands 10 times prior to drawing it up in the syringe (after allowing the vial to sit for 30 minutes at room temperature if the vial is stored in a refrigerator).