Amfucin Dosage/Direction for Use

Amphotericin B is given by intravenous infusion conventionally as a colloidal complex with sodium deoxycholate. Before starting therapy with any form of intravenous amphotericin B a test dose is usually advised and the patient observed carefully for about 30 minutes. An initial test dose (1 mg infused over 20 to 30 minutes) treatment usually starts with a daily dose of 250 mcg/kg, increased gradually to a maximum of 1 mg/kg daily; in seriously ill patients up to 1.5 mg/kg daily or on alternate days may be necessary. If treatment is stopped for longer than 7 days, it should be resumed at a dose of 250 micrograms/kg daily and increased gradually. The daily dose is infused over 2 to 4 hours at a concentration of 100 micrograms/mL in glucose 5%. Slower infusion, over up to 6 hours, may be necessary to reduce the incidence of acute toxic effects or as prescribed by the physician.
Directions for Reconstitution: Add 12 mL of sterile water for injection into the vial yielding 4mg/mL of amphotericin.
Shake the vial vigorously to completely disperse amphotericin. A yellow-colored suspension will be form.
Calculate amount of reconstituted amphotericin to be further diluted. Withdraw computed amount of reconstituted amphotericin into a sterile syringe.
Inject the syringe contents through into the appropriate amount of 5% Dextrose.
Amphotericin must be diluted with 5% Dextrose Injection to a final concentration of 1 to 2 mg/mL prior to administration. Lower concentrations (0.2 to 0.5 mg/mL) may be appropriate for infants and small children, so as to provide sufficient volume for infusion.
Note: Do not reconstitute with saline, add saline to the reconstituted solution nor mix with other drugs; presence of bacteriostatic agent in solution may cause precipitation. Reconstituted solution should be refrigerated (2 to 8°C) and use within 24 hours. Do not freeze. Discard any unused solution.